Phospho-GATA3(S308) Recombinant Rabbit mAb [KD验证]
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This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia.
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推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000
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本产品仅用作科学研究!


Flow cytometric analysis of Phospho-GATA3 (S308) expression in HT-1080 cells using Phospho-GATA3 (S308) antibody . Green, isotype control; red, Phospho-GATA3 (S308).

Immunocytochemical staining of HepG2 cells with phospho-GATA3 (S308) antibody . Nuclei were stained blue with DAPI; Phospho-GATA3 (S308) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium: Scale bar, 20 μm.

Western blotting analysis using Phospho-GATA3(S308) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-GATA3(S308) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Phospho-GATA3(S308) antibody . Phospho-GATA3(S308) expression in wild type (WT) and Phospho-GATA3(S308) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Phospho-GATA3(S308) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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Store at -20℃ for one year.
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