This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. Transcript variants derived from alternative promoter usage and/or alternative splicing exist; they encode different isoforms.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Oxysterol binding protein like 1A expression in HT-1080 cells using Oxysterol binding protein like 1A antibody . Green, isotype control; red, Oxysterol binding protein like 1A.

Immunocytochemical staining of HT-1080 cells with Oxysterol binding protein like 1A antibody . Nuclei were stained blue with DAPI; Oxysterol binding protein like 1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Oxysterol binding protein like 1A antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Oxysterol binding protein like 1A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Oxysterol binding protein like 1A antibody . Oxysterol binding protein like 1A expression in wild type (WT) and Oxysterol binding protein like 1A shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Oxysterol binding protein like 1A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Oxysterol binding protein like 1A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Oxysterol binding protein like 1A antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using Oxysterol binding protein like 1A antibody , Top panel: wild-type (WT); Bottom panal: Oxysterol binding protein like 1A shRNA knockdown (KD). Nuclei were stained blue with DAPI; Oxysterol binding protein like 1A was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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