This gene encodes a member of the tetratrico peptide repeat (TPR) family. The encoded protein is involved in cilium biogenesis. Mutations of a similar gene in mouse can cause polycystic kidney disease. Several transcript variants encoding distinct isoforms have been identified for this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Intraflagellar transport 88 expression in HeLa cells using Intraflagellar transport 88 antibody . Green, isotype control; red, Intraflagellar transport 88.

Immunocytochemical staining of HeLa cells with Intraflagellar transport 88 antibody . Nuclei were stained blue with DAPI; Intraflagellar transport 88 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Intraflagellar transport 88 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Intraflagellar transport 88 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Intraflagellar transport 88 antibody . Intraflagellar transport 88 expression in wild type (WT) and Intraflagellar transport 88 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Intraflagellar transport 88 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Intraflagellar transport 88 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Intraflagellar transport 88 antibody and analyzed using BD flow cytometer.

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