Enables calcium ion binding activity; non-membrane spanning protein tyrosine kinase activity; and protein serine/threonine kinase activity. Involved in apoptotic process; cell population proliferation; and protein autophosphorylation. Located in cell cortex and plasma membrane.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Immunocytochemical staining of HepG2 cells with Maternal embryonic leucine zipper kinase antibody . Nuclei were stained blue with DAPI; Maternal embryonic leucine zipper kinase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using maternal embryonic leucine zipper kinase antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with maternal embryonic leucine zipper kinase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using maternal embryonic leucine zipper kinase antibody . Maternal embryonic leucine zipper kinase expression in wild-type (WT) and maternal embryonic leucine zipper kinase (MELK) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with maternal embryonic leucine zipper kinase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Flow cytometric analysis of Maternal embryonic leucine zipper kinase expression in HepG2 cells using Maternal embryonic leucine zipper kinase antibody . Green, isotype control; red, Maternal embryonic leucine zipper kinase.

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