This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

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Flow cytometric analysis of Dimethylarginine dimethylaminohydrolase 1 expression in HT-1080 cells using Dimethylarginine dimethylaminohydrolase 1 antibody . Green, isotype control; red, Dimethylarginine dimethylaminohydrolase 1.

Western blotting analysis using dimethylarginine dimethylaminohydrolase 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dimethylarginine dimethylaminohydrolase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using dimethylarginine dimethylaminohydrolase 1 antibody . Dimethylarginine dimethylaminohydrolase 1 expression in wild type (WT) and dimethylarginine dimethylaminohydrolase 1 (DDAH1) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with dimethylarginine dimethylaminohydrolase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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