The protein encoded by this gene is an RNA-binding protein that regulates pre-mRNA splicing, export of mRNAs from the nucleus, protein translation, and mRNA stability. The encoded protein is involved in myelinization and oligodendrocyte differentiation and may play a role in schizophrenia. Multiple transcript variants encoding different isoforms have been found for this gene.

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Flow cytometric analysis of QKI expression in C2C12 cells using QKI antibody . Green, isotype control; red, QKI.

Immunocytochemical staining of C2C12 cells with QKI antibody . Nuclei were stained blue with DAPI; QKI was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using QKI antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with QKI antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using QKI antibody . QKI expression in wild type (WT) and QKI shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with QKI antibody and HRP-conjugated goat anti rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of QKI knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with QKI antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using QKI antibody , Top panel: wild-type (WT); Bottom panal: QKI shRNA knockdown (KD). Nuclei were stained blue with DAPI; QKI was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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