The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of ROCK2 expression in HepG2 cells using ROCK2 antibody . Green, isotype control; red, ROCK2.

Immunocytochemical staining of HepG2 cells with ROCK2 antibody . Nuclei were stained blue with DAPI; ROCK2 cochaperone 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using ROCK2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ROCK2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using ROCK2 antibody . ROCK2 expression in wild type (WT) and ROCK2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ROCK2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of ROCK2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ROCK2 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using ROCK2 antibody , Top panel: wild-type (WT); Bottom panal: ROCK2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; ROCK2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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