Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).

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Flow cytometric analysis of Chloride intracellular channel 4 expression in HAP-1 cells using Chloride intracellular channel 4 antibody . Green, isotype control; red, Chloride intracellular channel 4.

Immunocytochemical staining of HAP-1 cells with Chloride intracellular channel 4 antibody . Nuclei were stained blue with DAPI; Chloride intracellular channel 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Chloride intracellular channel 4 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Chloride intracellular channel 4 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using chloride intracellular channel 4 antibody . Chloride intracellular channel 4 expression in wild type (WT) and Chloride intracellular channel 4 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Chloride intracellular channel 4 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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