This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

本产品仅用作科学研究！

Flow cytometric analysis of NDRG1 expression in HT-1080 cells using NDRG1 antibody . Green, isotype control; red, NDRG1.

Immunocytochemical staining of HepG2 cells with NDRG1 antibody . Nuclei were stained blue with DAPI; NDRG1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using NDRG1 antibody . Total cell lysates (30 μ g) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDRG1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using NDRG1 antibody . NDRG1 expression in wild type (WT) and NDRG1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NDRG1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ ™ ECL Substrate Kit .

Store at -20℃ for one year.