The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.

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Flow cytometric analysis of Aly/REF export factor expression in HeLa cells using Aly/REF export factor antibody . Green, isotype control; red, Aly/REF export factor.

Immunocytochemical staining of Hela cells with Aly/REF export factor antibody . Nuclei were stained blue with DAPI; Aly/REF export factor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Aly/REF export factor antibody . Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Aly/REF export factor antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using Aly/REF export factor antibody . Aly/REF export factor expression in wild-type (WT) and Aly/REF export factor (ALYREF) knockdown (KD) HeLa cells with 25 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Aly/REF export factor antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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