This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

本产品仅用作科学研究！

Flow cytometric analysis of Dynamin 1 like expression in HepG2 cells using Dynamin 1 like antibody . Green, isotype control; red, Dynamin 1 like.

Immunocytochemical staining of HepG2 cells with Dynamin 1 like protein antibody . Nuclei were stained blue with DAPI; Dynamin 1 like was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using dynamin 1 like antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dynamin 1 like antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using dynamin 1 like antibody . Dynamin 1 like expression in wild type (WT) and dynamin 1 like (DNM1L) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with dynamin 1 like antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Dynamin 1 like knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Dynamin 1 like antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using Dynamin 1 like antibody , Top panel: wild-type (WT); Bottom panal: Dynamin 1 like shRNA knockdown (KD). Nuclei were stained blue with DAPI; Dynamin 1 like was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Store at -20℃ for one year.