The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses.

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Flow cytometric analysis of EIF2AK2 expression in HepG2 cells using EIF2AK2 antibody . Green, isotype control; red, EIF2AK2.

Immunocytochemical staining of HepG2 cells with EIF2AK2 antibody . Nuclei were stained blue with DAPI; EIF2AK2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using EIF2AK2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF2AK2 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit . EIF2AK2, eukaryotic translation initiation factor 2 alpha kinase 2.

Western blotting analysis using EIF2AK2 antibody . EIF2AK2 expression in wild-type (WT) and EIF2AK2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with EIF2AK2 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of EIF2AK2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with EIF2AK2 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using EIF2AK2 antibody , Top panel: wild-type (WT); Bottom panal: EIF2AK2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; EIF2AK2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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