Enables U6 snRNA binding activity. Involved in several processes, including cellular response to virus; positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator; and tumor necrosis factor-mediated signaling pathway. Located in annulate lamellae; cytoplasm; and nucleus. Part of nuclear pore. Biomarker of colorectal cancer.

推荐稀释比 WB: 1/2000-1/10000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Eukaryotic translation initiation factor 5A expression in HepG2 cells using Eukaryotic translation initiation factor 5A antibody . Green, isotype control; red, Eukaryotic translation initiation factor 5A.

Immunocytochemical staining of HepG2 cells with Eukaryotic translation initiation factor 5A antibody . Nuclei were stained blue with DAPI; Eukaryotic translation initiation factor 5A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using eukaryotic translation initiation factor 5A antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with eukaryotic translation initiation factor 5A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using eukaryotic translation initiation factor 5A antibody . Eukaryotic translation initiation factor 5A expression in wild type (WT) and eukaryotic translation initiation factor 5A (EIF5A) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with eukaryotic translation initiation factor 5A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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