The protein encoded by this gene belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. This protein binds and activates cyclin-dependent kinase 2 and thus promotes transition through G1/S and G2/M.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Cyclin A2 expression in HepG2 cells using Cyclin A2 antibody . Green, isotype control; red, Cyclin A2.

Immunocytochemical staining of HepG2 cells with Cyclin A2 antibody . Nuclei were stained blue with DAPI; Cyclin A2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Cyclin A2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cyclin A2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Cyclin A2 antibody . Cyclin A2 expression in wild type (WT) and Cyclin A2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Cyclin A2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of CyclinA2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with CyclinA2 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using Cyclin A2 antibody , Top panel: wild-type (WT); Bottom panal: Cyclin A2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Cyclin A2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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