Enables armadillo repeat domain binding activity; protein phosphatase 2A binding activity; and protein-macromolecule adaptor activity. Involved in negative regulation of hippo signaling. Part of FAR/SIN/STRIPAK complex.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Western blotting analysis using striatin 4 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with striatin 4 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using striatin 4 antibody . Striatin 4 expression in wild-type (WT) and striatin 4 (STRN4) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with striatin 4 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HAP-1 cells with Striatin 4 antibody . Nuclei were stained blue with DAPI; Striatin 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Validation of Striatin 4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Striatin 4 antibody and analyzed using BD flow cytometer.

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