This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.

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Flow cytometric analysis of Glucose-6-phosphate dehydrogenase expression in HepG2 cells using Glucose-6-phosphate dehydrogenase antibody . Green, isotype control; red, Glucose-6-phosphate dehydrogenase.

Immunocytochemical staining of HepG2 cells with Glucose-6-phosphate dehydrogenase antibody . Nuclei were stained blue with DAPI; Glucose-6-phosphate dehydrogenase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar, 20 μm.

Western blotting analysis using Glucose-6-phosphate dehydrogenase antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Glucose-6-phosphate dehydrogenase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using glucose-6-phosphate dehydrogenase antibody . Glucose-6-phosphate dehydrogenase expression in wild type (WT) and glucose-6-phosphate dehydrogenase (G6PD) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with glucose-6-phosphate dehydrogenase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Glucose-6-phosphate dehydrogenase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Glucose-6-phosphate dehydrogenase antibody and analyzed using BD flow cytometer.

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