Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

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Flow cytometric analysis of Neurofilament medium chain expression in Hela cells using Neurofilament medium chain antibody . Green, isotype control; red, Neurofilament medium chain.

Immunocytochemical staining of Hela cells with Neurofilament medium chain antibody . Nuclei were stained blue with DAPI; Neurofilament medium chain was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using neurofilament medium chain antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with neurofilament medium chain antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using neurofilament medium chain antibody . Neurofilament medium chain expression in wild-type (WT) and neurofilament medium chain (NEFM) knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with neurofilament medium chain antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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