The protein encoded by this gene is a mutase that catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Two transcript variants encoding different isoforms have been found for this gene.

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Western blotting analysis using Phosphoglycerate mutase 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phosphoglycerate mutase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Phosphogiycerate mutase 1 antibody . Phosphogiycerate mutase 1 expression in wild type (WT) and Phosphogiycerate mutase 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Phosphogiycerate mutase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Flow cytometric analysis of Phosphoglycerate mutase 1 expression in C2C12 cells using Phosphoglycerate mutase 1 antibody . Green, isotype control; red, Phosphoglycerate mutase 1.

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