The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme.

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Flow cytometric analysis of Pyrophosphatase 1 expression in HT-1080 cells using Pyrophosphatase 1 antibody . Green, isotype control; red, Pyrophosphatase 1.

Immunocytochemical staining of HT-1080 cells with Pyrophosphatase 1 antibody . Nuclei were stained blue with DAPI; Pyrophosphatase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Pyrophosphatase 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Pyrophosphatase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Pyrophosphatase 1 antibody . Pyrophosphatase 1 expression in wild type (WT) and Pyrophosphatase 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Pyrophosphatase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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