E-Cadherin is a 120 kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There, it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-cadherin/catenin complexes associate with corical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression.Tissue specificity: Non-neural epithelial tissues.

推荐稀释比 WB:1/1000-1/5000; IHC/1/200-1/500; ICC/IF:1/200-1/1000; FC:1/20

Western blot analysis of E Cadherin in PC-3 lysates using E Cadherin antibody.

Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using E Cadherin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunocytochemistry analysis of E Cadherin (green) in MCF-7 using E Cadherin antibody,and DAPI(blue)

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