Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

推荐稀释比 WB:1/500-1/1000; IHC:1/50-1/100; IF:1/50-1/200

Western blot analysis of Phospho-AKT (Ser473) in 293 lysates, treated with EGF or calf intestinal phosphatase (CIP)lysates using Akt (Phospho-Ser473) antibody.

Western blot analysis of Phospho-AKT (Ser473) in A549 cells, untreated or treated with EGF, using Akt (Phospho-Ser473) antibody (upper) and AKT(pan) antibody (lower).

Western blot analysis of Phospho-AKT (Ser473) in SKBR3 lysates treated with insulin and EGF,and pretreated with U126 and LY2942 lysates using Akt (Phospho-Ser473) antibody.

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using Akt (Phospho-Ser473) antibody High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide (lower).

Immunohistochemistry analysis of paraffin-embedded Human Lung carcinoma tissue using Phospho-AKT (Ser473) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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