Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE.

推荐稀释比 WB:1/500-1/1000;IHC:1/50-1/100;IP:1/20

Western blot analysis of BubR1 in CHO-K1, Ramos lysates using BUBR1 antibody.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。