Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the biosynthesis of serotonin by converting tryptophan to 5-hydroxy-L-tryptophan. Two isoforms of TPH exist: TPH-1 is mainly expressed in the periphery, whereas the expression of TPH-2 is restricted to neuronal cells and the central nervous system. Most of the serotonin found throughout the body is synthesized by TPH-1 in enterochromaffin cells of the gastrointestinal tract. Targeted disruption of the tph1 gene results in low levels of circulating and tissue serotonin.

推荐稀释比 WB:1/500-1/1000;IHC:1/50-1/100;IP:1/50

Western blot analysis of TPH1 in THP-1 lysates using Tryptophan 5 Hydroxylase 1 antibody.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。