May help to regulate cell structure and intracellular processes required for neural cell division and migration. Upon maturation of the brain, nestin expression is quickly down-regulated and replaced by expression of the neurofilament proteins. Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells.

推荐稀释比 IHC:1/50-1/100;IF:1/50-1/200

Immunohistochemistry analysis of paraffin-embedded Human kidney using Nestin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence analysis of Nestin in PC-12 using Nestin antibody.

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.