Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.

推荐稀释比 WB:1/500-1/1000;IHC:1/50-1/100;IF:1/50-1/200;ELISA:1/10000

Western blot analysis of Raf1 in HepG2 lysates using Raf1 antibody.

Western blot analysis of Raf1 in rat liver lysates using Raf1 antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded rat kidney tissue using Raf1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence analysis of Raf1 in mouse liver using Raf1 antibody(red),and DAPI (blue).

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