别名

Interleukin-10; IL-10; CSIF; IL10A; TGIF; B-TCGF; GVHDS; MGC126450; MGC126451

表达系统

 HEK293

表达区间

 Ser19-Asn178

分子量

The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Bis-Tris PAGE result.

内毒素

< 1.0 EU/μg by the LAL method.

纯度

> 95% ，Tris-Bis PAGE和 HPLC检测

生物活性

【ELISA Data】Immobilized Human IL-10, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 28.7ng/ml determined by ELISA.

【SPR Data】Human IL-10R alpha, His Tag immobilized on CM5 Chip can bind Human IL-10, His Tag with an affinity constant of 0.13 nM as determined in SPR assay.

制剂

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

外观

白色冻干粉

复溶方法

开盖之前，先离心。使用去离子水复溶至浓度不低于0.1 mg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。