Abstract

Bio-MOF-1 is a kind of metal-organic frameworks (MOFs) with bio-derived constitution, self-fabricated by zinc-adeninate secondary building units (SBUs) and may theoretically be endowed with biocompatible behaviors. Its biological applications currently contain several small molecule drugs delivery and in vitro mineralization, yet the related evidence on its biosafety and biocompatibility is still in great need. With the aid of self-modified protocol to synthesize micro/nanoscale bio-MOF-1 (m/n-bio-MOF-1), this study aimed to implement a systematic evaluation based on a series of tests from different dimensions including cell proliferation , oxidative stress, apoptosis and animal experiment, etc. These in vitro & in vivo biosafety and biocompatibility tests are to provide fresh evidence and advice for further applications. The outcomes indicated a concentration-dependent trend that 50 and 20 μg/mL groups proved biofriendly, with n-bio-MOF-1 surpassing m-bio-MOF-1. In conclusion, this work provided systematic evidence for the biosafety of m/n-bio-MOF-1, paved the way for their further exploration, and appealed more emphasis on MOFs toxicity.