IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription. DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries. NFHEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues. IL?33 was identified based on sequence and structural homology with IL?1 family cytokines. DVS 27, NF?HEV, and IL?33 share 100% amino acid sequence identity. IL?33 is constitutively expressed in smooth muscle and airway epithelia. It is up-regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL?1 alpha or IL?1 beta stimulation. Similar to IL, IL33 can be cleaved in vitro by caspase, generating an Nterminal fragment that is slightly shorter than the Cterminal fragment. The N?terminal portion of full length IL?33 contains a predicted bipartite nuclear localization sequence and a homeodomain?like helix?turn?helix DNA binding domain. By immunofluorescence, full length IL33 localizes to the nucleus in HUVECs and transfectants. The Cterminal fragment, corresponding to mature IL?33, binds and triggers signaling through mast cell IL?1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses. A ternary signaling complex is formed by the subsequent association of IL33 and ST2L with IL?1R AcP . Stimulation of Th2 polarized lymphocytes with mature IL33 in vitro induces IL?5 and IL?13 secretion. In vivo administration of mature IL?33 promotes increased production of IL5, IL13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues . Full length and mature human IL?33 share 52?58% aa sequence identity with mouse and rat IL?33. Human IL?33 shares less than 20% aa sequence identity with other IL?1 family proteins.

高纯度、高活性、低内毒素、高批间一致性

-25 ~ -15℃保存，收到货之后有效期1年。 复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。