Loading Control小鼠单抗试剂盒(HRP) Loading Control Mouse mAb Kit(HRP)小鼠单克隆抗体试剂盒（760010ES）

This kit is consist of three different but reliable antibodies which are specific to the most popular three loading control targets: GAPDH, β-Actin and β-Tubulin, also with three individual package.

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis, gene expression, and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer diseases.

推荐稀释比 WB:1/10000-1/20000

Western blot analysis of beta actin in Hela, 3T3, C6, CHO-K1 and COS7 lysates using beta actin antibody conjugated with HRP.

Western blot analysis of Tubulin beta in Hela, 3T3, C6, CHO-K1 and COS7 lysates using Tubulin beta antibody conjugated with HRP.

Western blot analysis of GAPDH in rat brain, CHO-K1 and HUVEC lysates using GAPDH antibody (HRP Conjugate).

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。