Abstract

Foodborne diseases caused by foodborne pathogens lead to serious harm to food safety and human health. This work fabricated an enzyme-responsive packaging film based on porous poly (lactic acid) (PLA) nanofibers modified by positively charged polyethyleneimine (PEI) and further to adsorb negatively charged pectin coating, which loaded with thymol (THY) for protecting fruits from microbial infection. The porous PLA nanofibers were fabricated by combining “Breath Figure” principle and electrospinning technique. The XPS and FTIR characterizations showed that PLA nanofiber membrane was successfully modified by PEI. The prepared nanofiber membrane significantly inhibited the growth of E. coli , S. aureus and Bacillus subtilis (＞95%), especially showed excellent antifungal activity against Aspergillus niger . The release of THY from pectin-coated porous nanofiber membrane ( [email protected] ) was triggered by pectinase, which was secreted by microorganisms from food contamination. Besides, the biocompatible [email protected] nanofiber membrane prolonged the shelf life of citrus. Therefore, this pectinase-responsive nanofiber membrane has desirable application prospects in the development of active or smart packaging systems.