CRISPR-Cas13a mediated nanosystem for attomolar detection of canine parvovirus type 2
Haroon Khan, Adeel Khan, Yufeng Liu, Su Wang, Sumaira Bibi, Hongpan Xu, Yuan Liu, Samran Durrani, Lian Jin, Nongyue He, Tao Xiong
Journal：CHINESE CHEMICAL LETTERS
IF：3.84
DOI：10.1016/j.cclet.2019.10.032
PMID：
Published：2019-10-31
research field：分子生物学纳米技术微生物学诊断方法环境生物学病毒学
Abstract
Graphical abstract The CPV-2 DNA extracted from dog’s intestine was amplified using RPA and transcribed into an RNA copy. The RNA copy was incubated with Cas13a and crRNA, upon activation collateral cleavage activity of Cas13a cleaves all the target RNA and nonspecific RNA in the vicinity producing fluorescent signals within 30 min.
