性能参数
蛋白别名(Synonyms) |
GCP2; PSMA; PSMAFGCP; FGCP; Folate hydrolase 1; FOLHNAALADase I; GCPII; GCPIImGCP; NAALAD1; NAALADase I; NAALAdase; FOLH1; GIG27; PSM; FOLH; mGCP |
种属(Species) |
Human |
表达系统(Source) |
HEK293 |
表达序列(Sequence) |
Lys44-Ala750 |
Accession |
Q04609-1 |
标签(Tag) |
N-His-Avi |
分子量(Molecular Weight) |
The protein has a predicted MW of 82.43 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Bis-Tris PAGE result. |
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
纯度(Purity) |
> 95% as determined by Bis-Tris PAGE; |
活性(Activity) |
ELISA Data: Immobilized Biotinylated Human PSMA, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. |
制剂(Formulation) |
Lyophilized from 0.22 μm filtered solution in 25 mM MES, 500 mM NaCl (pH 6.5). Normally 8% mannitol is added as protectant before lyophilization. |
外观(Appearance) |
冻干粉 |
储存条件
-25~-15℃保存,收到货之后有效期1年。复溶后, 2 ~8 °C保存,7天有效期。复溶后, -85~-65℃保存,3个月有效期。
复溶方法
开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。
注意事项
Ver. CN20240905