Recombinant Human KRAS G12V (HLA-A*11:01) Complex Protein,His-Avi Tag

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产品说明书

FAQ

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已发表文献

性能参数

 

表达区间及表达系统(Source)

Recombinant Human KRAS G12V (HLA-A*11:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*11:01),Ile21-

Met119(B2M) and VVGAVGVGK peptide.[Accession | AAV53343.1(HLA-A*11:01)&P61769

(B2M)&VVGAVGVGK]

分子量大小(Molecular Weight)

The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 56-65 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE

> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

 

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~-15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

 

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.           

 

Ver. CN20230526

400-6111-883