The 8th China (Shanghai) International Technology Fair (CSITF), which aims to promote advanced technology exchanges globally, is currently taking place in Shanghai World Expo Exhibition and Convention Center. This year, the technologies about the detection, control, and prevention of viral pathogens are undoubtedly among the hottest topics that have drawn massive attention. 

Empowered by our innovative enzyme modification technology, large-scale protein fermentation/purification technology, and highly-efficient antibody screening technology, Yeasen has been playing an important role in the battle against coronavirus by providing core enzymes for pathogen detection, vaccine production, and related research and applications.

Our UNICON® HotStart Taq DNA Polymerase is an antibody modified enzyme whose activity is blocked at room temperature and will only be restored after the antibodies are denatured and dissociate from the polymerase during the initial denaturation step of the PCR. This technology ensures high specificity and sensitivity of the PCR reactions, effectively reduces false positives and allows room-temperature reaction setup.

Messenger RNA vaccines against COVID-19 are widely used ever since the pandemic outbreak due to their safety, rapid development, and potent immunogenicity. The mRNA molecules designed to encode virus antigens are synthesized with phage RNA polymerases, 5’ modified with capping enzymes, and finally purified and isolated with DNase I and magnetic beads.

Yeasen offers GMP-grade enzymes and related products used for the production of mRNA vaccines. These products are manufactured and inventoried at GMP-grade and are suitable for various applications, including vaccine production and therapeutic products manufacturing.

UCF·ME^TM T7 RNA Polymerase (GMP-grade) is a recombinant enzyme obtained from Escherichia coli, which catalyzes in vitro RNA synthesis from NTP on DNA template downstream T7 promoters (5'-TAATACGACT CACTATAG*-3'). This T7 RNA Polymerase enables the synthesis of both short (<200nt) and long (500nt-4000nt) RNA transcripts with high promoter-specificity and high RNA yield.

UCF.ME^TM mRNA Vaccinia Capping Enzyme (GMP-grade) is a multi-functional mRNA-capping enzyme composed of two subunits (D1 and D12), exhibiting RNA 5’-triphosphatase activity, guanylyltransferase activity, and guanine methyltransferase activity. The enzyme enables the addition of 7-methylguanylate Cap-0 structure (m7Gppp) to the 5' end of RNA. Under proper conditions and with materials like reaction buffer, GTP and SAM, the capping enzyme can finish RNA capping within one hour, with the correct orientation, and with an efficiency of nearly 100%.

UCF·ME^TM mRNA Cap 2´-O-Methyltransferase (GMP-grade) is a recombinant protein obtained from an E. Coli strain that expresses the gene for the Vaccinia mRNA Cap 2’-O-Methyltransferase. The Cap-1 structure enhances the translation efficiency and reduces the immunogenicity of the mRNA. The enzyme adds a methyl group at the 2’-O position of the first nucleotide adjacent to the mRNA’s Cap-0 structure to form a Cap-1 structure. It requires SAM as the methyl donor and RNA with m7Gppp cap as the substrate.

UCF·ME^TM UltraNuclease is a nonspecific endonuclease that cleavages and digests DNA and RNA of all formats (double-stranded, single-stranded, linear, circular, naturally occurring, or denatured) under a broad range of experimental conditions. It has been widely used for nucleic acid removal for numerous applications.

With our dedicated R&D team's over 3 years of continuous refining, Yeasen has developed the gene-modified E. Coli-expressed UCF·ME^TM UltraNuclease, which is characterized by its exceptional purity and high nuclease activity. Manufactured under the GMP guideline, this product has no protease activity and is free of endotoxin or viral contaminants, thus can be applied for virus purification and vaccine manufacturing.

Yeasen Offering Enzymes, Modules, and Kits for NGS Library Preparations from DNA or RNA Samples

With our recently upgraded formulas, the Hieff NGS ® OnePot DNA Library Prep Kits exhibit even higher efficiencies in end-repair, A-tailing, and adapter ligation. The high-fidelity polymerases used in these upgraded kits significantly reduce error rates and GC bias, resulting in more accurate and even represented sequencing data.

Hieff NGS® Ultima Dual-mode RNA Library Prep Kit

Hieff NGS® MaxUp Human rRNA Depletion Kit（rRNA & ITS/ETS）

This kit removes cytoplasmic (18S, 28S, 5.8S, and human 45S ITS/ETS) and mitochondrial (12S and 16S) ribosomal RNA from human-derived total RNA samples. FFPE samples are usually associated with higher ITS/ETS rates than fresh tissue samples. As a kit focused on the effective rRNA-depletion especially for degraded samples, it contains the probes targeting the human 45S ITS/ETS regions, ensuring informative sequencing data for low-quality RNA samples.

DNA Purification Beads

 1）Easy Operation：A smooth transation can be applied if switching from A* XP, as all operation steps as well as the treated libraries' sizes and yields are the same

2）High purification and size-selection performances：High recovery rate (up to 90%), precise size-selection

3）Broad Applications：Can be applied for the clean-up or size-selection of FFPE or gDNA samples originated from fecal, blood, environmental samples, etc.

4）Broad Compatibility：Compatible with various library preparation kits

5）Stable Quality：No variations between batches

1×dsDNA HS Assay Kit for Qubit

1）Easy to use: Ready-to-use master mix

2）Highly sensetive：64× dilution，precise quantification

3）Highly stable: Flourescent signal lasts for 3 hours; accuracy won't be impacted after 20 days of room temperature storage.