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Validation of NEK6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with NEK6 antibody and analyzed using BD flow cytometer.
Flow cytometric analysis of NEK6 expression in HT-1080 cells using NEK6 antibody. Green, isotype control; red, NEK6.
Immunocytochemical staining of HT-1080 cells with NEK6 antibody . Nuclei were stained blue with DAPI; NEK6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using NEK6 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NEK6 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .
Western blotting analysis using NEK6 antibody . NEK6 expression in wild type (WT) and NEK6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with NEK6 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .
Immunocytochemical staining of HeLa cells using NEK6 antibody , Top panel: wild-type (WT); Bottom panal: NEK6 shRNA knockdown (KD). Nuclei were stained blue with DAPI; NEK6 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
