Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13E family of ribosomal proteins. It is located in the cytoplasm. This gene is expressed at significantly higher levels in benign breast lesions than in breast carcinomas. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; IHC-P: 1/100-1/200

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Flow cytometric analysis of Ribosomal protein L13 expression in C2C12 cells using Ribosomal protein L13 antibody . Green, isotype control; red, Ribosomal protein L13.

Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using ribosomal protein L13 antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.

Western blotting analysis using ribosomal protein L13 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ribosomal protein L13 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using ribosomal protein L13 antibody . Ribosomal protein L13 expression in wild-type (WT) and ribosomal protein L13 (RPL13) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ribosomal protein L13 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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